Äkta pure micro

With a low hold-up volume flow path, it’s your complete solution for micropreparative columns

With a low hold-up volume flow path, the ÄKTA pure micro struktur is your solution for small sample volumes and micropreparative columns. Combined with Fraction collector F9-T, the system can help you purify proteins when sample volumes are low – for example, when preparing for cryo-EM experiments. If you already have an ÄKTA pure 25 M system in your lab, you can use the Micro kit to omvandla the flow path for microscale purification. ÄKTA pure micro es equipped with special hardware components to optimize gradient performance ( mL mixer chamber), minimize sample dilution (tubing kit and micro injection valve) and to maintain peak volumes from UV to the fractionation tube (UV microflow fängelse, micro conductivity monitor, micro outlet valve and F9-T equipped with optional micro nozzle for drop-size of approx. 8µL). A fully optimized autentisk pure struktur is able to maintain the performance achieved bygd a Superdex increase /30 or Capto HiRes 5/50 column as demonstrated in the chromatograms.

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  • äkta pure micro

    • ÄKTA Pure Chromatography System

    • Application supported : Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T. 
    • Autosampler injection : Optional
    • Buffer selection : Upgradeable to 14 inlet
    • Compliance : The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva. 
    • Dimensions (W × H × D) : × × mm
    • Weight (excluding computer, sample pump, fraction collector) : Up to 53 kg
    • Power consumption : VA (typical), 25 VA (power-save)
    • Protection class : IP 21 
    • System pump type : Piston pump, metering type 
    • Flow rate :  

               ÄKTA pure 25 and ÄKTA pure micro: to 25 mL/min (up to 50 mL/min during column packing) 

               ÄKTA pure to mL/min (up to mL/min during column packing)

    • Operating Pressure range :

         

    Liquid chromatography system ÄKTA pure™

    for protein purificationUVmodular

    ӒKTA pure™ chromatography system allows for quick and easy customization due to its modular design. Choose ÄKTA pure™ 25 with a flow rate of up to 25 mL/min or ÄKTA pure™ with a flow rate of up to mL/min. We offer more than 20 hardware options for your custom system, including a new UV monitor. Introducing ӒKTA pure™ T the first dual wavelength, flexible chromatography system With 2 fixed wavelengths at and nm ӒKTA pure™ T offers a new way of addressing your complex purification needs. ӒKTA pure™ T chromatography systems are available in 25 mL/min and mL/min formats. It retains the flexibility and additional module selection options as ӒKTA pure™ L and M. If you have an existing ӒKTA pure™ L chromatography system, it can be upgraded with the U9-T UV monitor to perform a broader set of applications. Get started with ÄKTA pure™ — free online course Access on-demand training to learn how to prepare and use the ÄKTA pure™ chromatography system. Our online learning is divided into several short topics and includes instructive images and video tutorials for protein purification using ÄKTA pure™.